The effect of hyaluronidase on stem cells yielding from infranatant fraction of lipoaspirate

Assem HK Abdelhafiz, Mohamed Makboul, Ahmed M Tohamy, Fatma Yassin, Ehab Ragab


The aim of our study was to evaluate the effect of adding hyaluronidase to the wetting solution used in liposuction on stem cell content and amount in the infranatant fluid of liposuction as a simple and easy way for stem cells isolation. This study was conducted on lipoaspirate obtained from patients asking for body contouring by liposuction. A hundred specimens from the infranatant fluid fraction of the lipoaspirate were included in our study. A high statistical significant difference with a P<0.05 was found in the number of viable adipose-derived stem cells (ADSCs) isolated from infranatant of lipoaspirate by using hyaluronidase (33.5U/ml) compared with that without using it (11.47U/ml). The infranatat fluid of lipoaspirate being a rich source of ADSCs shouldn’t be discarded. Time factor using infranatant fluid is much less than that consumed using digestive enzymes from fat layer and also it is more economic. Adding hyaluronidase to the wetting solution increases the yield of isolated stem cells compared to not adding it.


Stem cell; ADSC; regenerative medicine

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