Age-related changes in membrane fluidity and fluorescence intensity by tachykinin neuropeptide NKB and Aβ (25-35) with 17β estradiol in female rat brain

Rashmi Jha, Abbas Ali Mahdi, Shivani Pandey, Najma Zaheer Baquer, Sudha Mahajan Cowsik

Abstract


     

Changes in the fluidity of membrane lipids are known to occur during aging and by lipid peroxidation. It is well documented that the fluidity state of the lipid phase in a membrane is important for the activity of intrinsic membrane proteins. Oxidants and fluidity of membrane lipids play a significant role in aging and age related neurodegenerative diseases. The aim of the present study was to determine the effect of tachykinin neuropeptide, Neurokinin B (NKB) and Amyloid beta fragment Aβ (25-35) on 17β estradiol (E2) treated aging female rat synaptosomes of different age groups. Aging brain functions were measured by membrane fluidity and fluorescent intensity with neuropeptides. An in-vitro incubation of Aβ (25-35) in E2 treated brain synaptosomes showed toxic effects on all the parameters. These effects of aging and Aβ (25–35) on membrane fluidity were restored by NKB and combined NKB and Aβ (25–35) with E2. Furthermore, we measured the Tryptophan (Trp) fluorescence to monitor changes in proteins and to make inferences regarding structure and dynamics. Trp is a sensitive marker of protein oxidation and its fluorescence significantly increased in E2 treated synaptosomes of aging rats. Furthermore, to evaluate the effect of oxidative stress on the membrane and protein conformation, fluorescent probe 1-Anilino-8-Naphthalenesulfonate (ANS) were used. An increase in ANS fluorescence in E2 treated synaptosomes of aging rats indicated that E2 is associated with significant conformational changes and surface hydrophobicity of membranes and proteins.


Keywords


Aging, Neurokinin B, Amyloid Beta (25 - 35), Estradiol

Full Text:

PDF

References


Harman D. Role of free radicals in aging and disease. Ann N Y Acad Sci 673:126-41, 1992.

Baquer NZ, Taha A, Kumar P, McLean P, Cowsik SM, Kale RK, Singh R, Sharma D. A metabolic and functional overview of brain aging linked to neurological disorders. Biogerontology 10: 377–413, 2009.

Brann DW, Krishnan D, Chandramohan W, Virendra BM, Mohammad MK. Neurotrophic neuroprotective actions of estrogen: basic mechanism and clinical implications. Steroids 72: 381-405, 2007.

Kumar P, Taha A, Kale RK, Cowsik SM, Baquer NZ. Physiological and biochemical effect of 17β estradiol in aging female rat brain. Exp. Gerontol 46: 597-605, 2011.

Moorthy K, Yadav SUC, Mantha AK, Cowsik SM, Sharma D, Baquer NZ. Effect of estradiol and progesterone treatment on lipid profile in naturally menopausal rats from different age groups. Biogerontology 5: 1–9, 2004.

Henderson VW. Action of estrogens in the aging brain: dementia and cognitive aging. Biochem. Biophys. Acta 1800: 1077–1083, 2010.

Gronemeyer H, Gustafsson JA, Laudet V. Principles for modulation of the nuclear receptor superfamily. Nature Rev Drug Disc 3: 950-964, 2004.

Spencer JL, Waters EM, Romeo RD, Wood GE, Millner TA, McEwen BS. Uncovering the mechanism of estrogen effects on hippocampal function. Front Neuroendocrinology 29: 219-237, 2008.

Garcia-Segura LM, Azcoitia I, DonCarlos LL. Neuroprotection by estradiol. Prog. Neurobiol 63: 29–60, 2001.

Almeida TA, Rojo J, Nieto PM, Pinto FM, Hernandez M, Martin JD, Candenas ML. Tachykinins and tachykinin receptors: structure and activity relationships. Curr Med Chem 11: 2045- 2081, 2004.

Patacchini R, Lecci A, Holzer P, Maggi CA. Newly discovered tachykinins raise new questions about their peripheral roles and the tachykinin nomenclature. Trends Pharmacol Sci 25:1-3, 2004.

Mantha AK, Moorthy K, Cowsik SM, Baquer NZ. Neuroprotective role of neurokinin B (NKB) on Amyloid β (25–35) induced toxicity in aging rat brain synaptosomes: involvement in oxidative stress and excitotoxicity. Biogerentology 7 (1): 1–17, 2006.

Mantha AK, Moorthy K, Cowsik SM, Baquer NZ. Membrane associated functions of Neurokinin B (NKB) on Aβ (25–35) induced toxicity in aging rat brain synaptosomes. Biogerentology 7 (1): 19-33, 2006.

Turska E, Lachowicz L, Wasiak T. Effect of analogues of substance P fragments on the MAO activity in rat brain. Gen Pahrmacol 16(3): 293 -5, 1985.

Kowall NW, Beal MF, Buscigliot J, Duffyt LK, Yankner NW. An in vivo model for the neurodegenerative effects of β amyloid and protection by substance P. Neurobiology 88: 7247-7251, 1991.

Yankner BA, Duffy LK, Kirschner DA. Neurotrophic and neurotoxic effects of amyloid protein: Reversal by tachykinin neuropeptides. Science 250: 279–282, 1990.

Page NM, Lowry PJ (2000). Is 'pre-eclampsia' simply a response to the side effects of a placental tachykinin? J Endocrinol 167(3): 355-361, 2000.

Wenk GL, Rance NE, Mobley SL. Effects of excitatory amino acid lesions upon neurokinin B and acetylcholine neurons in the nucleus basalis of the rat. Brain Res 679(1): 8-14, 1995.

Wenk GL, Zajaczkowski W, Danysz W. Neuroprotection of acetylcholinergic basal forebrain neurons by memantine and neurokinin B. Behav Brain Res 83(1-2): 129-133, 1997.

Yamaguchi H, Maat-Schieman ML, Duinen SG, Prins FA, Neeskens P, Natte R , Roos RA. Amyloid beta protein (Abeta) starts to deposit as plasma membrane-bound form in diffuse plaques of brains from hereditary cerebral hemorrhage with amyloidosis-Dutch type, Alzheimer disease and nondemented aged subjects. J Neuropathol Exp Neurol 59 (8): 723-732, 2000.

Dyson HJ, Wright PE. Defining solution conformations of small linear peptides. Annu Rev Biophys Biophys Chem 20: 519-538, 1991.

Kaiser ET, Kezdy FJ. Peptides with affinity for membranes. Annu Rev Biophys Biophys Chem 16:561-581, 1987.

Dohlman HG, Thorner J, Caron MG, Lefkowitz RJ. Model systems for the study of seven-transmembrane-segment receptors. Annu Rev Biochem 60: 653-688, 1991.

Kleinert HD, Rosenberg SH, Baker WR, Stein HH, Klinghofer V, Barlow J, Spina K, Polakowski J, Kovar P, Cohen J. Discovery of a peptide-based renin inhibitor with oral bioavailability and efficacy. Science 257(5078): 1940-1943, 1992.

Kremer JJ, Sklansky DJ, Murphy RM. Profile of changes in lipid bilayer structure caused by beta-amyloid peptide. Biochemistry 40(29): 8563-8571, 2001.

Eckert GP, Cairns NJ, Muller WE. Piracetam reverses hippocampal membrane alterations in Alzheimer's disease. J Neural Transm 106(7-8): 757-761, 1999.

Ptitsyn OB, Pain RH, Semistonov GV, Zerovnik E, Razqulyaev OI. Evidence for a molten globule state as a general intermediate in protein folding. FEBS Lett 262 (1): 20-4, 1990.

Semistonov GV, Rodionova NA, Razaqulyaev OI, Uversky VN, Gripas AF, Glimanshin RI. Study of the “molten globule” intermediate state in protein folding by a hydrophobic fluorescent probe. Biopolymers 1991; 31(1): 119-28.

Jha R, Pandey S, Mahdi AA, Baquer NZ, Cowsik SM. Neuroprotective Role of 17β estradiol with Tachykinin Neuropeptide NKB and Aβ (25-35) in aging female rat brain. Advances in Aging Research 2 (4): 130-136, 2013.

Moorthy K, Yadav UCS, Siddiqui MR, Basir SF, Sharma D, Baquer NZ. Effect of estradiol and progesterone treatment on carbohydrate metabolizing enzymes in tissues of aging female rats. Biogerontology. 5: 249–259, 2004a.

Mayanil CS, Kazmi SM, Baquer NZ. Na+, K+-ATPase and Mg2+-ATPase activities in different regions of rat brain during alloxan diabetes. J Neurochem 39: 903–908, 1982.

Lebel CP, Schatz RA. Altered synaptosomal phospholipid metabolism after toluene: possible relationship with membrane fluidity, Na+, K+-adenosine triphosphatase and phospholipid methylation. J Pharmacol Exp Ther 253: 1189–1197, 1990.

Babusikova E, Hatok EJ, Dobrota ED, Kaplan EP. Age-related Oxidative Modifications of Proteins and Lipids in Rat Brain. Neurochem Res 32:1351–1356, 2007.

Dousset N, Ferretti G, Taus M, Valdiquie P, Curatola G. Fluorescence analysis of lipoprotein peroxidation. Methods Enzymol 233:459–469, 1994.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254, 1976.

Muller WE, Kirsch C, Eckert GP. Membrane-disordering effects of beta-amyloid peptides. Biochem Soc Trans 29(4): 617-623, 2001.

Geula C, Wu CK, Saroff D, Lorenzo A, Yuan M, Yankner BA. Aging renders the brain vulnerable to amyloid beta-protein neurotoxicity. Nat Med 4(7): 827-831, 1998.

Sham YY, I Muegge, A Warshel. The effect of protein relaxation on charge-charge interactions and dielectric constants of proteins. Biophys. J 74:1744 –1753, 1998.

Warshel A. Computer modeling of chemical reactions in enzymes and solutions. Wiley-Interscience New York, NY, 1991.

Warshel A, J Aqvist. Electrostatic energy and macromolecular function. Annu. Rev. Biophys. Biophys. Chem 20:267–298, 1991.

Beechem JM, L Brand. Time-resolved fluorescence of proteins. Annu. Rev. Biochem 54:43–71, 1985.

Eftink MR. Fluorescence techniques for studying protein structure. Methods Biochem. Anal 35:127–205, 1991.

Lakowicz J. Principles of Fluorescence Spectroscopy. Plenum Publishers, New York, 1991.

Turner DC, Brand L. Quantative estimation of protein binding site polarity. Fluorescence of N-arylaminonaphthalenesulfonates. Biochemistry 7(10): 3381-90, 1968.


Refbacks

  • There are currently no refbacks.


Best Quality Medical Supplies is a registered company in Untied States specializing in sales of medical equipment and supplies. We are a distributor for hospitals, doctor offices, medical clinics, nursing schools, and nursing homes within
the United States. We also provide services for overseas clients. Click here for details and orders.




My website has truly excellent support, going well beyond what's expected from the customer. Check them out here.