A comparison of urine microscopy and urine culture results of patients considered to have urinary tract infection

Cemal Polat, Osman Evliyaoğlu, Revşa Evin Canpolat Erkan, Ahmet Baştürk, Tugrul Çakir, Arif Aslaner, Şükran Can, Nuriye Mete


Urinary tract infections are the diseases of the urogenital system caused by various microorganisms. Currently, the most used descriptive tests are urine strips, microscopic analysis of urine and spot bacteruria tests. The aim of this study was to present the consistency of culture results with leukocyte count determined cytometrically in the urine of patients considered to have a urinary tract infection and thereby facilitate treatment approaches. A retrospective examination was performed with the urine samples of patients sent to central laboratory of the Dicle University Hospital in January2012- December 2013. Both microscopic urine analysis and urine culture were performed for each patient on the same day. The study comprised a total of 839 patients; 222 males and 617 females. Urine culture results and urine microscopy findings of patients with positive urine strip tests were compared. With the comparison of urine culture results, positive urine microscopy findings were found to have 92%sensitivity, 26% specificity, 52% positive predictive value (PPV)and 78% negative predictive value (NPV) (p<0.001).Compared to the culture results, the urine microscopy findings of patients with positive urine strip tests were found to have high sensitivity and low specificity (p<0.001). As a result empirical antimicrobial therapy can be considered for patients with positive urine microscopy findings without waiting for culture results, and patients with negative results are recommended to have urine culture results.


Urinary tract infection, leukocyte number, sensitivity, specificity, microscopy

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Singh S. Urine examination: Its importance in the pediatric medicine. Indian J Pediatr58:717-723, 1991.

Lane DR, Takhar SS. Diagnosis and management of urinary tract infection and pyelonephritis. Emerg Med Clin North Am 29:539-552, 2011.

Hellerstein S. Urinary tract infections.Old and new concepts. Pediatr Clin North Am 42:1433-1457, 1995.

Boscia JA, Abrutyn E, Levison ME, Pitsakis PG, Kaye D. Pyuria and asymptomatic bacteriuria in elderly ambulatory women. Ann Intern Med110:404-405, 1989.

.Lunn A, Holden S, Boswell T, Watson AR. Automated microscopy, dipsticks and the diagnosis of urinary tract infection. Arch Dis Child 95:193-197, 2010.

Ducharme J, Neilson S, Ginn JL. Can urine cultures and reagent test strips be used to diagnose urinary tract infection in elderly emergency department patients without focal urinary symptoms? CJEM 9:87-92, 2007.

Falbo R, Sala MR, Signorelli S, Venturi N, Signorini S, Brambilla P. Bacteriuria screening by automated whole field image based microscopy reduces the number of nece- ssary urine cultures. J Clin Microbiol 50:1427-1429, 2012.

Kim SY, Kim YJ, Hwang SH, Kim HH, Son HC, Lee E. Evaluation of the Sysmex UF-100 urine cell analyzer as a screening test to reduce the need for urine cultures for community-acquired urinary tract infection.Am J Clin Pathol 128:922-925, 2007.

Steinmetz J, Henny J, Gueguen R. Etablissement de limites de référence des sédiments urinaires mesurés sur UF-50TM Sysmex. Ann BiolClin 62:671-680, 2004.

Simerville JA, Maxted WC, Pahira JJ.Urinalysis: a comprehensive review. Am Fam Physician 71:1153-1162, 2005.


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